divided incubation trays pepperchips  (PEPperPRINT gmbh)

Journal: Cells

Article Title: Comparison of Monoclonal Gammopathies Linked to Poliovirus or Coxsackievirus vs. Other Infectious Pathogens

doi: 10.3390/cells10020438

Figure Lengend Snippet: Results of PEPperCHIP ® Infectious Epitope MicroArrays obtained for Monoclonal IgGs Specific for VP1 Coat Protein of Human Polioviruses.Microarrays were incubated with the patient monoclonal IgG (50 or 100 µg/mL), stained with secondary and control antibodies, then read out at scanning intensities of 7/7 (red/ green) (see Materials and Methods). Antibody response against peptides is annotated next to corresponding signals in the intensity plot (left panel). Well-defined staining of HA control peptides appear in green; PV control peptides appear in red.

Article Snippet: Microarrays were placed in suited PEPperCHIP ® incubation trays (PEPperPRINT GmbH, Heidelberg, Germany).

Techniques: Incubation, Staining, Control

Journal: Cells

Article Title: Comparison of Monoclonal Gammopathies Linked to Poliovirus or Coxsackievirus vs. Other Infectious Pathogens

doi: 10.3390/cells10020438

Figure Lengend Snippet: Results of PEPperCHIP ® Infectious Epitope MicroArrays obtained for Monoclonal IgGs Specific for VP1 Coat Protein of Human Coxsackieviruses. Microarrays were incubated with the patient monoclonal IgG (50 or 100 µg/mL), stained with secondary and control antibodies, then read out at scanning intensities of 7/7 (red/green) (see Materials and Methods). Antibody response against peptides is annotated next to corresponding signals in intensity plots (left panels). Well-defined staining of HA control peptides appear in green, PV control peptides appear in red.

Article Snippet: Microarrays were placed in suited PEPperCHIP ® incubation trays (PEPperPRINT GmbH, Heidelberg, Germany).

Techniques: Incubation, Staining, Control

Journal: Cells

Article Title: Comparison of Monoclonal Gammopathies Linked to Poliovirus or Coxsackievirus vs. Other Infectious Pathogens

doi: 10.3390/cells10020438

Figure Lengend Snippet: PEPperCHIP ® Infectious Epitope MicroArray Results of monoclonal gammopathy of undetermined significance (MGUS)/smoldering myeloma (SM) Monoclonal IgGs.

Article Snippet: Microarrays were placed in suited PEPperCHIP ® incubation trays (PEPperPRINT GmbH, Heidelberg, Germany).

Techniques: Microarray, Sequencing

Journal: Cells

Article Title: Comparison of Monoclonal Gammopathies Linked to Poliovirus or Coxsackievirus vs. Other Infectious Pathogens

doi: 10.3390/cells10020438

Figure Lengend Snippet: Results of the “PV/CVB” Dot Blotting Assays obtained for the 6 Monoclonal Igs found to be Specific for the PALTAV/AETG Epitopes with the PEPperCHIP ® Infectious Epitope Arrays. Membranes were spotted with the following peptides: ( a ) HP-1a, HP-3a and HC-a, respectively relevant to PV1, PV3 and CVB1/B3; ( b ) irrelevant HP-1b, HP-3b and HC-b peptides; ( c ) irrelevant HP-1c, HP-3c and HC-c peptides (see ), then incubated with positive or negative controls or samples of serum from patients. After validation that no signal was obtained with irrelevant peptides for serum samples, purified monoclonal Igs were incubated with relevant peptides only. Immunoblot revelation with secondary antibodies (Materials & Methods). The assay confirmed that the purified monoclonal IgG of MGUS patients 4_38, 4_51 and 4_52 (in bold, left panel) bound specifically to PALTAVETG or PALTAAETG epitopes.

Article Snippet: Microarrays were placed in suited PEPperCHIP ® incubation trays (PEPperPRINT GmbH, Heidelberg, Germany).

Techniques: Incubation, Biomarker Discovery, Purification, Western Blot